Din sökning

SERVA Fluo-610 Standard I is a ready-to-use fluorescent labelled protein marker for direct detection in a SDS-PAGE gel or on a membrane in Western Blotting. The marker proteins in the range from 14.4 kDa to 9.4 kDa are labelled with the highly sensitive and stable fluorescent dye Lightning Red. SERVA Lightning Red (cat. no. 43402) is a fluorescent dye for rapid labelling in minutes of proteins prior to SDS PAGE and/or Western Blotting, making any staining and washing steps after electrophoresis unnecessary.

Lightning Red is a fluorescent dye for rapid labeling of proteins prior to SDS PAGE and/or Western blotting, eliminating the need for any staining and washing step after electrophoresis. In Western blotting, the labeled protein samples can be easily used for sample normalization using the total protein signal as a loading control. The dye is fully compatible with mass spectrometry and other subsequent methods.

• Easy and fast labeling prior to SDS PAGE and Western blotting: qualitative analysis in 2-5 min, quantitative analysis 30 min
• Wide protein concentration range: for complex and for purified samples from 1 µg/ml to 20 mg/ml
• No protein concentration determination, purification or concentration steps necessary before or after labeling
• No staining or washing steps after electrophoresis
• Very high sensitivity, very low background
• Wide dynamic and linear range
• Detection by fluorescence imaging device

Highly sensitive fluorescent dye for detection of proteins in e. g. SDS PAGE, native PAGE or 2D gels. The dye does not interfere with immunodetection. Therefore you can stain your gel with SERVA ProteinStain Fluo-R and then proceed with Western Blotting receiving a copy of your gel on the membrane.
The dye can as well be used for pre-staining, just use it in the loading buffer instead of bromophenol blue or other dyes.
It is as sensitive as silver staining, but has superior staining properties, which makes it the first choice for proteomic research. The dye has a good linearity, high contrast and is compatible with MS/MS analysis. The staining can be as well combined with silver staining and DIGE.
Easily soluble in water. The dye is best excited with UV light of wave length 473/488 nm. Excitation with laser light of wave length 532 nm is as well possible, but less sensitive.

Total protein stain for non-denaturing and denaturing 1D and 2D gel electrophoresis and blotting. The dye reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product (ʎ?Ex 518 nm, ʎ?Em 610 nm). SERVA Purple HiSens shows highest sensitivity compared to most other protein stains. Even difficult to stain proteins as glycoproteins and lipoproteins can be accurately detected.

• Environmentally friendly, easy to use
• Sensitivity to as low as <20 pg/band
• Linear quantification over 4 orders of magnitude
• Compatible with MS, DIGE-labelling
• After imaging gel can be further processed by Western Blotting

Assay based on the eco-friendly fluorescent dye SERVA Purple. The dye reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely red-fluorescent product. The assay exhibits very low protein binding variation, leading to more accurate protein concentration values.

• Fast and simple - no heating and reduction steps, completed in 1 h
• Compatible with many detergents and reducing agents
• Accurate staining of glyco-, phospho-, hydrophobic proteins and peptides
• Single tube and 96- or 384-well-format for high-thoughput
• Detection limit of 100 ng/ml for peptides and 40 ng/ml for proteins
• Linear quantification over 3 orders of magnitude
• Compatible with downstream applications like 1D- and 2D-PAGE, MS, DIGE-labelling, HPLC

Silver staining kit for easy and rapid staining of proteins after SDS PAGE:
Contains everything needed for fixation and staining.

• Fast staining procedure (45 - 60 min.)
• Very low background
• High sensitivity
• With detailed staining manual
• For 25 applications

To determine the molecular weight of proteins from SDS-PAGE on a
polyacrylamide gel, SERVA offers a range of different types of
pre-stained and unstained protein markers from natural and recombinant origin.
The VisiBlot Standard I has three pre-stained protein bands and seven bands
with IgG binding sites for visualization via antibody detection.


These ready-to-use standards are perfectly suitable as protein markers for your SDS-PAGE. Pre-stained markers are convenient for controlling the separation during the electrophoresis run or the efficiency of protein transfer to a membrane. Since the molecular weight of the proteins differs from that of the unstained markers due to the staining, unstained protein standards are used for accurate molecular weight determination.

SERVA unstained protein standard IV:

• Precise molecular weight determination in SDS-PAGE gel
• Testing of transfer efficiency in Western Blotting

• Tracking of protein separation in the gel
• Estimation of protein sizes

VisiBlot Standard I is a mixture of 10 recombinant proteins of a molecular weight range from 25 kDa to 150 kDa. Protein bands of 25 kDa, 45 kDa and 85 kDa are prestained allowing monitoring of protein separation during SDS PAGE. The remaining five proteins contain several IgG binding sites. Hence marker proteins bind to primary or secondary antibodies used in Western Blotting facilitating easy marker visualization on the transfer membrane. Because the proteins have no chromophore attached, the marker enables accurate molecular weight estimation.
Recommended loading volume for a mini gel is 5 µl/lane.

• Ready-to-use, no reconstitution, further dilution or heating required
• Prestained bands for monitoring electrophoresis and membrane transfer
• Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods
• Molecular weight determination of proteins detected on transfer membrane