The DMEM medium is a modification of the BME which contains a higher concentration of amino acids, vitamins and other additives. DMEM is for supporting and maintaining a vast range of mammalian cell types. There are two types of DMEM: one with a high glucose content (4.5g/L), and the other with a low glucose content (1.0g/L). The DMEM low glucose has been developed for the culture of mouse embryonic cells.

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemia cells.

Minimum Essential Medium (MEM) is a modification of Eagle’s earlier medium Basal Medium Eagle (BME). This MEM w/ Hanks’ Salts formulation contains Hank’s salts for use without CO2.

The DMEM Ham’s F12 offers excellent performance for certain epithelial, endothelial and granulose cell types. With proper supplementation, it is a highly successful basic media for serum free cell culture.

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO2 gas.

Protect your cell lines from contamination by foreign organisms such as bacteria, fungi and viruses and/or use the antibiotics for selection after transfection.
Most antibiotics suppress the growth of micro-organisms by blocking an anabolic pathway.
Nanomycopulitine actively kills bacteria, including mycoplasma, in all three stages of development without interfering with the eukaryotic metabolism.

Iscoves media are enriched modifications of DMEM containing sodium selenite. They are excellent for rapidly proliferating high-density cell cultures. The addition of BSA, purified human transferring and soybean lecithin creates a serum free condition ideal for supporting B and T lymphocytes. IMDM was the first media utilizing HEPES buffer. Other cell types can be cultured using this medium under serum free or reduced serum conditions.

BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten.

Mc Coy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

This complex medium was developed specifically for nutritional research of chicken
fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.
The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.