Lightning Red is a fluorescent dye for rapid labeling of proteins prior to SDS PAGE and/or Western blotting, eliminating the need for any staining and washing step after electrophoresis. In Western blotting, the labeled protein samples can be easily used for sample normalization using the total protein signal as a loading control. The dye is fully compatible with mass spectrometry and other subsequent methods.

• Easy and fast labeling prior to SDS PAGE and Western blotting: qualitative analysis in 2-5 min, quantitative analysis 30 min
• Wide protein concentration range: for complex and for purified samples from 1 µg/ml to 20 mg/ml
• No protein concentration determination, purification or concentration steps necessary before or after labeling
• No staining or washing steps after electrophoresis
• Very high sensitivity, very low background
• Wide dynamic and linear range
• Detection by fluorescence imaging device